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38 Konferencji Polskiego Towarzystwa Fykologicznego.
Influence of Low Temperatures on Growth of Ice and Arctic Microalgae
Aleksandra Klassa1, Julia Skowera1, Aleksandra Warzocha1, Szymon Zajączkowski1, Filip Pniewski2, Aleksandra Zgrundo2
1III Liceum Ogólnokształcące im. Marynarki Wojennej RP w Gdyni, ul. Legionów 27, 81-405 Gdynia,
2Uniwersytet Gdański, Instytut Oceanografii, Al. Piłsudskiego 46, 81-378 Gdynia,
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The group of students from III LO is preparing the stratospheric mission within 3-SAT project (http://3inspace.com/). In the study, the influence of low temperatures simulating stratospheric conditions on growth of ice and arctic microalgae was examined.
For the experiment 6 strains of green algae and 2 strains of diatoms were selected. They were isolated to monocultures from rocks, macroalgae or bryophytes collected in Svalbard and from snow in Tatra mountains (strain CCALA 970).
The strains were grown on agar-F2 medium plates for 2 weeks in a temperature-controlled growth chamber at 12ºC. Then 10 mm in diameter discs were cut out and transferred into 5.0 ml Eppendorf tubes. For each strain four tubes were placed for two hours (the estimated duration of the stratospheric balloon flight) at -80ºC, four at -20ºC and four were left as a control. Subsequently, to all tubes 2 ml of the F2 culture medium were added and then they were placed in the growth chamber again. Quantitative analysis was made in a Bürker chamber after 8 and 12 days following standard procedure.
The reactions of strains to low temperatures were various. For one diatom strain only the temperature of -20ºC induced its growth but for the other one both temperatures increased the number of cell (-80ºC even two fold). For four strains of green algae the temperature of -20ºC stimulated their growth, even two fold, whereas -80ºC had limiting effect. For the remaining strains the temperature of -20ºC limited their growth, while -80ºC doubled the number of cells.
The results of the experiments suggest that studied microalgae would survive in stratospheric temperature gradient, but to assess their survival potential the influence of other environmental variables as ultraviolet radiation and pressure should also be tested.